Journal: Scientific reports

Article Title: Complete omission of exon 21 from Slc12a2 transcripts in mice results in hearing loss.

doi: 10.1038/s41598-025-99827-7

Figure Lengend Snippet: Fig. 8. Minigene assay of exon 21 of Slc12a2. (A) Minigene structures. Raus sarcoma virus long terminal repeat (LTR), exon2 and 3 of rat insulin-2 (rnIns2), M13 forward sequencing primer (M13F) and M13 reverse primer (M13R), partial genomic region of wild-type or Em2 allele of Slc12a2 including exon 21, and polyA signal are shown. Numbers in blue indicate the nucleotide lengths (bp) of the Slc12a2 genomic region inserted into the vector. Gray arrows indicate primer sets to amplify reporter transcripts. (B) RT-PCR to detect reporter transcripts in HEK293T cells. Inserted alleles with nucleotide at c.2912 are indicated above the image of the RT-PCR transcripts. Lane 7; 100 bp ladder marker.

Article Snippet: The resulting product was inserted between M13 forward and M13 reverse sequencing primer regions in the pSpliceExpress vector (Addgene #32485)58.

Techniques: Mini Gene Assay, Virus, Sequencing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Marker